Hemoglobin electrophoresis in acrylamide gel.

By THOMAS G. FERRIS, ROBERT E. EASTERLING AND RICHARD E. BUDD T HE VARIOUS types of hemoglobins have been identified and reported by means of zone electrophoresis using supporting media such as filter paper,’ starch,2 agar,3 cellulose acetate,4 and combinations of these media. Recently a synthetic acrylamide gel ( Cyanogum 41#{176} ) has been investigated and found to have characteristics which, in several respects, make it superior to other media for the electrophoretic separation of the serum proteins.57 Raymond7 mentioned that the gel was a satisfactory medium for the separation of hemoglobins. A technic and use of this acrylamide gel for the electrophoretic separation of hemoglobins in one hour is presented.